Western Science Center 305
For the past two decades, the application of genetic dissection and molecular biology has resulted in an explosion in our knowledge of the mechanisms that control the process of Development. One of the major experimental systems that contributed to this explosion is the model organism Drosophila melanogaster. My laboratory is studying two aspects of the molecular basis of the body plan. The body plan is required for positioning and determining the identity of the various body parts.
The first aspect of the body plan that we study is the role that the protein encoded by the gene fushi tarazu plays in determining the number of segments of Drosophila body plan. Fushi tarazu protein is expressed in every other segment resulting in bands of Fushi tarazu expression across the anterior posterior axis. Without Fushi tarazu protein, the embryo develops lacking half of its segments.
The second aspect of the body plan that we study is the role of the proteins encoded by the two genes, proboscipedia and Sex combs reduced, in determining of the identity of four body parts. Both proboscipedia and Sex combs reduced are homeotic genes. Mutant alleles in homeotic genes result in striking phenotypes where one body part is transformed into the likeness of another. Loss-of-Proboscipedia protein results in the transformation of the mouth parts into a pair of first leg tarsi.
Sivanantharajah, L., and Percival-Smith, A. 2015. Differential pleiotropy and HOX functional organization. Dev. Biol. 398: 1-10.
Sivanantharajah, L., and Percival-Smith, A. 2014. Acquisition of a Leucine zipper motif as a mechanism of antimorphy for an allele of the Drosophila Hox gene Sex combs reduced. G3 (Bethesda) 4: 829-838.
Kanca, O., Caussinus, E., Denes, A. S., Percival-Smith, A., and Affolter, M. 2014. Raeppli: a whole tissue labeling tool for live imaging of Drosophila development. Development 141: 472-480.
Ukada, H., Percival-Smith, A., and Sinclair, B. J. 2013. Increased abundance of Frost mRNA during recovery from cold stress is not essential for cold tolerance in adult Drosophila melanogaster. Insect Mol Biol 22: 541-550
Camiletti, A. L., Percival-Smith, A., and Thompson, G. J. 2013. Honey bee mandibular pheromone inhibits ovary development and fecundity in a fruit fly. Entomologia Experimentis et Applicata 147:262-268