Our research program on genetic variation including human molecular genetics deals with genetic and epigenetic factors associated with complex diseases and phenotypes with a focus on neurogenomics. It using whole genome based methods (microarrays; expressed, SNPs and methylation specific oligonucleotide microarrays and complete genome sequences) in studies on Schizophrenia, Alcoholism, Fetal Alcohol Syndrome and Cancers. They involve human families including monozygotic twins discordant for the disease and mouse as the genetic animal model. The results obtained have offered a novel insight, particularly the importance of epigenetics and gene regulation in the dissection of behaviors. This novelty is recognized by the genetics community including granting agencies (NSERC, NAMI (NIH), CIHR, ATCG, OMHF, SSO, etc.) who have funded this research fully and adequately for over 35 years.
Bio 4950a: Seminar in Genetics
Bio 4583a: Investigative Techniques in Genetics
Most Recent Ph.D. Graduates
Katarzyna Mantha (Ph.D. 2013) Currently, Faculty, Lambton College
Haroon Sheikh (Ph.D. 2014) Currently – CIHR PDF, University of Iowa, Ames
Morgan Kleiber (Ph.D. 2015) Currently – CIHR PDF, University of California, San Diego
Raihan Uddin (Ph.D. 2015) Currently – Laboratory Supervisor, Department of Biology, Western university
Ben Laufer (Ph.D. 2016) Currently – CIHR PDF, University of California, Davis
Most Recent Publications
Chater-Diehl, EJ, Benjamin I. Laufer, Christina A. Castellani, Bonnie L. Alberry, Shiva M. Singh (2016). Alteration of gene expression, DNA methylation, and histone methylation in free radicalscavenging networks in adult mouse hippocampus following fetal alcohol exposure. PLoS One. PMID: 27136348
Alberry B. and SM Singh (2016). Developmental and behavioral consequences of early life maternal separation stress in a mouse model of FASD. Behavioural Brain Research 308:
Castellani, C., M G Melka, JL Gui, R O’Reilly and SM Singh (2015). Integration of DNA sequence and DNA methylation changes in two MZ twin pairs discordant for Schizophrenia. Schizophrenia Research 169:433-440.
Castellani, CA., Melka*, MG., Gui*, JL., Gallo*, AJ., O’Reilly, RL., Singh, SM. (2016). Genomes of monozygotic twins discordant for schizophrenia delineate de novo features of twin differences and disease threshold. Translational Psychiatry (Submitted).
Castellani, C., Melka, M., Gui JL, O'Reilly, R and SM Singh. (2015). Integration of DNA Sequence and DNA Methylation Changes in Two Monozygotic Twin Pairs Discordant for Schizophrenia. Schizophrenia Research 169:433-440.
Laufer, B and S.M. Singh. (2015). Strategies of precision modulation of gene expression by epigenome editing: An Overview. Epigenetics and chromatin. PMID: 26388942
Laufer, B., Kapalanga, J., Castellani*, C.A., Diehl*, E.J., Yan, L., and Singh, S.M.(2015). Associative DNA Methylation Changes in Children with FASD. Epigenomics:1-16.
Ouellette, SJ, E Russell, KR Kryski, H Sheikh, S Singh, G Koren. (2015). Hair cortisol concentrations in higher- and lower-stress mother-daughter dyads: A pilot study of associations and moderators. Developmental psychobiology. PMID: 25820649
Melkaye G. Melka*, Christina A. Castellani*, Richard O’Reilly and Shiva M. Singh. (2015). Insights into the origin of DNA methylation differences between monozygotic twins discordant for Schizophrenia. Journal of Molecular Psychiatry. PMID: 26137221
Ben Laufer, Shiva M. Singh. (2015). Strategies for precision modulation of gene expression by epigenome editing: an overview. Epigenetics and Chromatin 8: 34.
Castellani, Benjamin I Laufer, Melkaye G Melka, Eric J Diehl, Richard L O'Reilly and Shiva M Singh. (2015). DNA methylation differences in monozygotic twin pairs discordant for schizophrenia identifies psychosis related genes and networks. BMC Med Genomics 8:17.
Castellani, Melka, Diehl, Laufer, O'Reilly, Singh. (2015). DNA methylation in psychosis: insights into aetiology and treatment. Epigenomics 7:67-74.
Melka MG, Rajakumar N, O'Reilly R, Singh SM. (2015). Olanzapine induced DNA methylation in the hippocampus and cerebellum in genes mapped to human chromosome 22q11 and implicated in schizophrenia. Psychiatric Genetics. PMID: 25415458
A more comprehensive list of publications from the Singh Lab can be seen on PubMed